A Comparison of AVIRIS and Landsat for Land Use Classification at the Urban Fringe

In this study we tested whether AVIRIS data allowed for improved land use classification over synthetic Landsat ETM+ data for a location on the urban-rural fringe of Colorado. After processing the AVIRIS image and creating a synthetic Landsat image, we used standard classification and post-classification procedures to compare the data sources for land use mapping. We found that, for this location, AVIRIS holds modest, but real, advantages over Landsat for the classification of heterogeneous and vegetated land uses. Furthermore, this advantage comes almost entirely from the large number of sensor spectral bands rather than the high Signal-to-Noise Ratio (SNR)

Estimating the Creation and Removal Date of Fracking Ponds Using Trend Analysis of Landsat Imagery

Hydraulic fracturing, or fracking, is a process of introducing liquid at high pressure to create fractures in shale rock formations, thus releasing natural gas. Flowback and produced water from fracking operations is typically stored in temporary open-air earthen impoundments, or frack ponds. Unfortunately, in the United States there is no public record of the location of impoundments, or the dates that impoundments are created or removed. In this study we use a dataset of drilling-related impoundments in Pennsylvania identified through the FrackFinder project led by SkyTruth, an environmental non-profit. For each impoundment location, we compiled all low cloud Landsat imagery from 2000 to 2016 and created a monthly time series for three bands: red, near-infrared (NIR), and the Normalized Difference Vegetation Index (NDVI). We identified the approximate date of creation and removal of impoundments from sudden breaks in the time series. To verify our method, we compared the results to date ranges derived from photointerpretation of all available historical imagery on Google Earth for a subset of impoundments. Based on our analysis, we found that the number of impoundments built annually increased rapidly from 2006 to 2010, and then slowed from 2010 to 2013. Since newer impoundments tend to be larger, however, the total impoundment area has continued to increase. The methods described in this study would be appropriate for finding the creation and removal date of a variety of industrial land use changes at known locations

Migrant Farmworkers\u27 Perceptions of Pesticide Risk Exposure in Adams County, Pennsylvania: A Cultural Risk Assessment

Agricultural exceptionalism, a system in which regular labor laws and standards do not apply to farm labor, makes migrant farmworkers particularly vulnerable populations—economically, socially, and in terms of environmental health. To address inequities inherent in migrant farmworker margin­aliza­tion, studies advocate for actively engaging the migrant farmworker population in the conversation surrounding these issues. We conducted 40 semi­structured interviews with migrant farmworkers in Adams County, Pennsylvania, to understand pesti­cide risk exposure perceptions and practices. We employed the Health Belief Model as our cultural risk assessment frame, using it in combination with technical risk assessment, which uses government calculations (from the Environmental Protection Agency) to quantify pesticide risk exposure. We used mixed methods analyses (quantitative and qualitative) to compare and understand farmworker demographics, perceived risk, perceived control, and risk behavior. Results show that demo­graphics —e.g., age, education, visa status—are important factors in risk perception. They also confirm observations present in many earlier studies. While trainings and educational materials are valuable to help build awareness of risk, a systemic lack of control over their circumstances make it hard for migrant farmworkers to engage in safe behavior. Results also highlight the limitations of technical risk assessment. Such calculations, however, rarely account for risk perceptions and experiences of farm­workers themselves. Acknowledging the voices of migrant farmworkers is an essential first step in rebalancing inequities of power in our food systems, and cultural risk assessment can help frame recommendations that target different stake­holders across the pesticide regulatory spectrum to ensure migrant farmworker needs and safety

Climate Change Perceptions, Data, and Adaptation in the Garhwal Himalayas of India

Himalayan communities that depend on rain-fed agriculture are disproportionately vulnerable to climate change. In this study, we compare local perceptions of climate change from a household survey (n = 251) to climate data obtained from the Global Land Data Assimilation System (GLDAS 2.1) and MODIS Terra Snow Cover data product datasets. The study is situated in and around the Kedarnath Wildlife Sanctuary, which is located within the Garhwal Himalayas in the Indian state of Uttarakhand. We found that a large majority of respondents perceive that rainfall is increasing and that snowfall is decreasing, while a smaller majority perceives an increase in summer temperatures and no change in winter temperatures. Agreeing with the perceptions of the majority, the climate data show an increase in summer temperature and winter rainfall. However, the climate data also show an increase in winter temperature, and no monotonic change in snowfall, findings which are contrary to the perception of the majority. Household perceptions of climate change were not associated with adaptation; while many households perceived change, very few reported that they were planning to adapt. To encourage adaptation, communities would benefit from locally appropriate climate data products, and collaboration on best practices with researchers, NGOs, and extension services

Recruitment Facilitation and Spatial Pattern Formation in Soft-Bottom Mussel Beds

Mussels (Mytilus edulis) build massive, spatially complex, biogenic structures that alter the biotic and abiotic environment and provide a variety of ecosystem services. Unlike rocky shores, where mussels can attach to the primary substrate, soft sediments are unsuitable for mussel attachment. We used a simple lattice model, field sampling, and field and laboratory experiments to examine facilitation of recruitment (i.e., preferential larval, juvenile, and adult attachment to mussel biogenic structure) and its role in the development of power-law spatial patterns observed in Maine, USA, soft-bottom mussel beds. The model demonstrated that recruitment facilitation produces power-law spatial structure similar to that in natural beds. Field results provided strong evidence for facilitation of recruitment to other mussels—they do not simply map onto a hard-substrate template of gravel and shell hash. Mussels were spatially decoupled from non-mussel hard substrates to which they can potentially recruit. Recent larval recruits were positively correlated with adult mussels, but not with other hard substrates. Mussels made byssal thread attachments to other mussels in much higher proportions than to other hard substrates. In a field experiment, mussel recruitment was highest to live mussels, followed by mussel shell hash and gravel, with almost no recruitment to muddy sand. In a laboratory experiment, evenly dispersed mussels rapidly self-organized into power-law clusters similar to those observed in nature. Collectively, the results indicate that facilitation of recruitment to existing mussels plays a major role in soft-bottom spatial pattern development. The interaction between large-scale resource availability (hard substrate) and local-scale recruitment facilitation may be responsible for creating complex power-law spatial structure in soft-bottom mussel beds

International Veterinary Epilepsy Task Force Consensus Proposal: Outcome of therapeutic interventions in canine and feline epilepsy

Common criteria for the diagnosis of drug resistance and the assessment of outcome are needed urgently as a prerequisite for standardized evaluation and reporting of individual therapeutic responses in canine epilepsy. Thus, we provide a proposal for the definition of drug resistance and partial therapeutic success in canine patients with epilepsy. This consensus statement also suggests a list of factors and aspects of outcome, which should be considered in addition to the impact on seizures. Moreover, these expert recommendations discuss criteria which determine the validity and informative value of a therapeutic trial in an individual patient and also suggest the application of individual outcome criteria. Agreement on common guidelines does not only render a basis for future optimization of individual patient management, but is also a presupposition for the design and implementation of clinical studies with highly standardized inclusion and exclusion criteria. Respective standardization will improve the comparability of findings from different studies and renders an improved basis for multicenter studies. Therefore, this proposal provides an in-depth discussion of the implications of outcome criteria for clinical studies. In particular ethical aspects and the different options for study design and application of individual patient-centered outcome criteria are considered

Characterization of an Nmr Homolog That Modulates GATA Factor-Mediated Nitrogen Metabolite Repression in Cryptococcus neoformans

Nitrogen source utilization plays a critical role in fungal development, secondary metabolite production and pathogenesis. In both the Ascomycota and Basidiomycota, GATA transcription factors globally activate the expression of catabolic enzyme-encoding genes required to degrade complex nitrogenous compounds. However, in the presence of preferred nitrogen sources such as ammonium, GATA factor activity is inhibited in some species through interaction with co-repressor Nmr proteins. This regulatory phenomenon, nitrogen metabolite repression, enables preferential utilization of readily assimilated nitrogen sources. In the basidiomycete pathogen Cryptococcus neoformans, the GATA factor Gat1/Are1 has been co-opted into regulating multiple key virulence traits in addition to nitrogen catabolism. Here, we further characterize Gat1/Are1 function and investigate the regulatory role of the predicted Nmr homolog Tar1. While GAT1/ARE1 expression is induced during nitrogen limitation, TAR1 transcription is unaffected by nitrogen availability. Deletion of TAR1 leads to inappropriate derepression of non-preferred nitrogen catabolic pathways in the simultaneous presence of favoured sources. In addition to exhibiting its evolutionary conserved role of inhibiting GATA factor activity under repressing conditions, Tar1 also positively regulates GAT1/ARE1 transcription under non-repressing conditions. The molecular mechanism by which Tar1 modulates nitrogen metabolite repression, however, remains open to speculation. Interaction between Tar1 and Gat1/Are1 was undetectable in a yeast two-hybrid assay, consistent with Tar1 and Gat1/Are1 each lacking the conserved C-terminus regions present in ascomycete Nmr proteins and GATA factors that are known to interact with each other. Importantly, both Tar1 and Gat1/Are1 are suppressors of C. neoformans virulence, reiterating and highlighting the paradigm of nitrogen regulation of pathogenesis

Basic science232. Certolizumab pegol prevents pro-inflammatory alterations in endothelial cell function

Background: Cardiovascular disease is a major comorbidity of rheumatoid arthritis (RA) and a leading cause of death. Chronic systemic inflammation involving tumour necrosis factor alpha (TNF) could contribute to endothelial activation and atherogenesis. A number of anti-TNF therapies are in current use for the treatment of RA, including certolizumab pegol (CZP), (Cimzia ®; UCB, Belgium). Anti-TNF therapy has been associated with reduced clinical cardiovascular disease risk and ameliorated vascular function in RA patients. However, the specific effects of TNF inhibitors on endothelial cell function are largely unknown. Our aim was to investigate the mechanisms underpinning CZP effects on TNF-activated human endothelial cells. Methods: Human aortic endothelial cells (HAoECs) were cultured in vitro and exposed to a) TNF alone, b) TNF plus CZP, or c) neither agent. Microarray analysis was used to examine the transcriptional profile of cells treated for 6 hrs and quantitative polymerase chain reaction (qPCR) analysed gene expression at 1, 3, 6 and 24 hrs. NF-κB localization and IκB degradation were investigated using immunocytochemistry, high content analysis and western blotting. Flow cytometry was conducted to detect microparticle release from HAoECs. Results: Transcriptional profiling revealed that while TNF alone had strong effects on endothelial gene expression, TNF and CZP in combination produced a global gene expression pattern similar to untreated control. The two most highly up-regulated genes in response to TNF treatment were adhesion molecules E-selectin and VCAM-1 (q 0.2 compared to control; p > 0.05 compared to TNF alone). The NF-κB pathway was confirmed as a downstream target of TNF-induced HAoEC activation, via nuclear translocation of NF-κB and degradation of IκB, effects which were abolished by treatment with CZP. In addition, flow cytometry detected an increased production of endothelial microparticles in TNF-activated HAoECs, which was prevented by treatment with CZP. Conclusions: We have found at a cellular level that a clinically available TNF inhibitor, CZP reduces the expression of adhesion molecule expression, and prevents TNF-induced activation of the NF-κB pathway. Furthermore, CZP prevents the production of microparticles by activated endothelial cells. This could be central to the prevention of inflammatory environments underlying these conditions and measurement of microparticles has potential as a novel prognostic marker for future cardiovascular events in this patient group. Disclosure statement: Y.A. received a research grant from UCB. I.B. received a research grant from UCB. S.H. received a research grant from UCB. All other authors have declared no conflicts of interes

Local and Global Analysis of Fragmentation in a Colorado Mountain Region

Although relationships between fragmentation of urban development and other forms of administrative and land cover fragmentation are important, they are poorly understood. This research aimed to better understand these relationships in order to inform land use planning in the Roaring Fork/Colorado River Corridor of Colorado. Change in fragmentation of urban development between 1985 and 1999 was modeled as a function of other forms of administrative and land cover fragmentation using two different regression specifications. While a standard “global” regression provided a good averaged model of change for the entire study area, a geographically weighted regression (GWR) demonstrated how the process changed locally over space. Results of the global regression showed that the intercept was close to zero and therefore the fragmentation of urban development was expected to be close to zero in the absence of other forms of fragmentation. Results of the GWR showed that the relationships between change in fragmentation of urban development and other fragmentation variables (initial edge density of urban development, edge density of public/private interface, farmland density and road density) varied significantly within the study area. By modeling this variation, GWR helped to identify ways to reduce fragmentation of urban development in two different regions of the study area. The analysis suggested that fragmentation of urban development in one area, Aspen–Basalt, was more strongly driven by amenity-related variables, while in the New Castle area fragmentation was more strongly driven by infrastructure-related variables. Ultimately, local analysis may help fine-tune “one-size-fits-all” land use polices for specific regions

The Wildland-Urban Interface: Evaluating the Definition Effect

The wildland‐urban interface (WUI) is the area where human-built structures and infrastructure abut or mix with naturally occurring vegetation types. Wildfires are of particular concern in the WUI because these areas comprise extensive flammable vegetation, numerous structures, and ample ignition sources. A priority of federal wildland fire policy in the United States is to help protect communities threatened by wildfire, creating a demand for maps of the WUI. In this study, five models of the WUI are compared for four counties in the United States. The models are all based on the widely cited characteristics of the WUI published in the Federal Register, although they differ slightly in their focus (vegetation or housing) and implementation (the details of the WUI definition). For models that differ in focus, I describe how the purpose of the map led to different results. For conceptually similar models, I assess how different effects—the “dasymetric effect,” the “settlement representation effect,” and the “merging buffer effect”—influence the extent of the WUI in different counties. The differences between the WUI maps can be more or less pronounced depending on the spatial distribution of housing, vegetation, and public land. No single mapping approach is unequivocally superior, and each has tradeoffs that need to be fully understood for use in management